Research:
PROPAGATION: HORMONE EFFECTS ON SEED (04RP_03)


Study Plan: A study of various Trillium species germination when treated with various hormones (Cytokinins, IBA, GA-3).

Objective 1: Conduct experiments to demonstrate any short term and long term effects(benefits or detrements) when seed is treated with various hormones (Cytokinins, IBA, GA-3).


Study Findings: (In Process).
- Pictures 1-17 (summer 2005) illustrate several test in process.

-Pictures 20-22 show some seeds growth(22 oct 2005) for Trillium reliquum experiment for test started on 12 July 2005. Pictures 24-25 show progress 0n 9 Nov 2005.

Scientific Name: Trillium Reliquum
Common Name(s): Fragrant White Toadshade
Location: SE USA
Description: Test shows 2 containers (1 treated, 1 control) to see the effects of GA-3 on Trillium Reliquum seed. Both were H2O2 treated seed with arils removed. Seed were soaked in 500ppm GA-3 solution overnight and placed on paper towel (also soaked with 500ppm GA-3 solution). Control container seed was H2O2 treated and placed on paper towel soaked with H2O.

Photograph: Harold Holmes

Scientific Name: Trillium Reliquum (Ga-3 treated)
Common Name(s): Fragrant White Toadshade
Location: SE USA
Description: Picture shows seed germination progress on 9 Nov 2005. Seed test commenced on 12 July 2005. Seeds were cleaned and H2O2 treated and soaked in 500ppm Ga-3 solution, then placed on damp towel (also wet with additional Ga-3 solution) in a 9X9 inch plastic container in a 70deg F. environment. Only differences between this and the Control was the Ga-3 application and the additional moisture due to the Ga-3 solution added to the paper towel. Note: long primary root at this stage.

Photograph: Harold Holmes

Scientific Name: Trillium Reliquum (not Ga-3 treated)
Common Name(s): Fragrant White Toadshade
Location: SE USA
Description: Picture shows seed germination progress on 9 Nov 2005. Seed test commenced on 12 July 2005. Seeds were cleaned and H2O2 treated, then placed on damp towel in a 9X9 inch plastic container in a 70deg F. environment. This test was used as a Control for the Ga-3 experiment. Note: primary root not long at this stage.

Photograph: Harold Holmes

Below are Pictures from a variety of experiments using Ga-3, IBA, and CY(cytokinins). In time I hope to show side by side comparisons of progress and results.

01_2005
02_2005
03_2005
04_2005

05_2005
06_2005
07_2005
08_2005
09_2005
10_2005(control)
17_2005(control)
20_2005 - large
21_2005 - large
22_2005- large
24_2005- Ga-3
25_2005- Ga-3
27_2005 - large
29_2005- large
26_2005- Ga-3 large
28_2005- Ga-3 large
40_C01_reliquum_13Dec05 large
41_C02_reliquum_13Dec05 large
42_C02_reliquum_13Dec05 large
43_C02_reliquum_13Dec05 large

44_C01-02_reliquum_13Dec05 -large
blank
blank
blank
C01_reliquum_2Jan06
C01_reliquum_2Jan06
C02_reliquum_GA3_2Jan06
C02_reliquum_GA3_2Jan06

C01_reliquum_19 Jan06
C02_reliquum_GA3_19 Jan06
blank
blank

Scientific Name: Trillium Flexipes (Cytokinins treated)
Common Name(s): Bent Trillium
Location: SE USA
Description: Picture on left shows seed germination progress on 22 Nov 2005. Seed test commenced on July 2004. Seeds were cleaned and H2O2 treated, then soaked in a Cytokinins solution and planted in an outside seedbed with a sand/ leaf mulch/perilite mix and Note: seed germinated and had blade leaves in spring 2005. Note primary root length. Photo #6 above shows seed bed in spring 2005 with blade leaves.

Photograph: Harold Holmes